FURTHER NOTES ON A TRYPANOSOME. 
135 
cess and the ci’eature takes on the typical Trypanosome 
facies. The only point about this that calls for special atten- 
tion is the development of the flagellum. 
It may be noted in passing that, owing to the flattening of 
the Trypanosomes prepared by the dry method, certain details 
may be more distinctly visible in such specimens than in those 
prepared by the wet method. 
The earliest stage of the development of the flagellum, of 
which one can be quite certain, is shown in fig. 2. Here it 
will be seen that two little projections have grown out from 
the kinetonucleus which is itself in process of division. These 
little structures sometimes take the Heidenhain rather deeply ; 
they are not, however, very easy to make out as any obliquity 
in the position of the kinetonucleus is apt to obscure them. 
Later stages are shown in figs. 3 — 5. Here the flagellum 
appears as a thick strand arising from a granule with not 
very definite contours, which is in turn attached to the kineto- 
nucleus. This granule at the origin of the flagellum is the 
blepharoplast. Tlie minute detail is not very clear in the wet 
preparations, but as far as I can make out the blepharoblast 
seems to be attached to the kinetonucleus by a double thread. 
This may be seen in much later stages (figs. 5 and 6). The 
blepharoplast seems like the kinetonucleus to be ultimately a 
thin rod-shaped body. It seems to arise from the kineto- 
nucleus, but I do not think in the light of its behaviour 
with the various stains that it is a chromatic body. It is true 
it often stains a deep sharp black with iron-hsematoxylin, but 
that is no test for chromatiu. It takes on a grey-blue pale 
colour with Helafield and shows up only dimly when at all with 
Fuchsin. In Twort’s combination it stains green like the 
flagellum and is not very clear or precise. I am therefore 
inclined to regard the blepharoplast and flagellar apparatus 
which grows out from it as achromatic. Our knowledge of 
achromatic nuclear elements is so limited at present that it is 
impossible to say whether they can be regarded as an expres- 
sion of the achromatic elements of the kinetonucleus or not. 
Oiemsa’s stain presents a greatly exaggerated picture of 
