FURTHEB NOTES UN A TRYPANOSOME. 
137 
condensations at the extreme points of the spindle, and the 
chromatin seems to pass along the “fibres”^ from the two 
main central masses in such a stage, as fig. 13, to either poles 
of the spindle. There is at a slightly later stage (figs. 14 and 
15), often a curious double appearance in the nuclei. Figs. 
IG and 17 give the final stages. The spindle persists for 
some time after the nuclei are reformed, and can be clearly 
seen as a bright line in the live specimens. 
Division occurs at all the different stages of development. 
There is an occasional not very well defined tendency to 
multiple division. Ecpial division is the rule, but unequal 
division is sometimes met with. Considerable variation in 
the division of the protoplasm is seen, as shown in figs. 
18 — 24. It will be observed that in figs. 20 — 22 the division 
of the protoplasm has started at the posterior end instead of 
the anterior, as is more usual. Specimens of this type were 
watched for many hours in the live state in the hope that 
they might be individuals conjugating, but no evidence in 
favour of this was forthcoming, and I was led to believe that 
they are in all probability mei’ely cases of division. 
Some very curious appearances were observed where the 
protoplasm had split into several rod-like processes. This is 
shown in fig- 24, and, while not a common appearance, is 
still far too frequently seen to be dismissed as a casual 
abnormality. The figures, I may say, hardly do justice to 
all the varying sizes and shapes to be seen at this stage in 
the development. 
As I have already said in a recent paper ^ on T. vittatae 
from the soft tortoise Emyda vittata, we seem to have in 
these forms a type of life-history which is probably very 
wide.spread. The rounding off process and the subsequent 
development of a flagellate stage occurs, as has been recently 
shown in many of the Trypanosomes from cold-blooded hosts. 
' I use the word fil>re quite without prejudice. The word con- 
veniently expresses the optical effect, and I have no means of knowing 
what they actually represent. 
- ‘ Quai-t. .lourn. Micr. Sci.,’ vol. .5.3, part 4. 
VOL. 54, PART 1 . — NEW SERIES. 1 0 
