574 
MARGARET RORIXSOX. 
not seen a single case of scissi parity. The buds (fig. 10) 
consist entirely of chromatin, and it is not until some time 
after their escape from the parent karyosome that the inner 
layer (plastin) makes its appearance in them. 
On first noticing these internal buds I was puzzled as to 
how they made their escape, but soon came to the conclusion 
that an exit was made as occasion demanded and then closed 
up again. I was therefore muck pleased to find that 
Schneider had described the same kind of internal budding 
in his account of Klossia (Aggregata) eberthi as long 
ago as 1883. See also Schellack (1907). 
At present, affinity for different stains is our usual criterion 
for differentiating the contents of the cell, and we make a 
broad distinction between chromatin and cytoplasm by sayiiig 
that one is basophile and the other acidophile. 
In working at this gregarine my first staining operation 
was generally the nse of Heidenhain’s hmmatoxylin, and I 
could not help noticing that in staining strongly with an acid 
stain, after using Heidenhain, the inner part of the karyo- 
some, the linin ineshwork and the centrosomes all took up 
the acid stain (eosin, orange (x., or picric and Licht-Griin), 
appearing to be stained by that and by nothing else. The 
centrioles and outer layer of the karyosomes and the chro- 
mosomes, however, kept black and were not affected by the 
acid stain at all. If I stained weakly with the acid stain the 
meshwork, inner part of the karyosome, and the centrosomes 
all retained the black stain of the Heideidiain, though the 
black on the inner part of the kai’yosome might with more 
accuracy be called grey. I could, in fact, vary the amount of 
greenness or blackness by varying the intensity of my acid 
stain, but one or other always predominated. Now the linin 
meshwork is known to consist partly of protoplasm and partly 
of chromatin. In the inner pa it of the karyosome chromatin 
is being converted into plastin, presumably for the nourish- 
ment of the nucleus and ultimately of the cytoplasm. Does 
it not seem that Avhile this process of conversion in going on, 
there must be in the inner part of the karyosome a mixture of 
