EEPEODUCTION OF KALFIDOEHYNCHUS AEENICOL/E. 575 
acidophile plastin and basophile cliromatin, and tliat in the 
centrosonies also there is a mixture of chromatin and an 
acidophile substance ? On this supposition we can, at least, 
give our explanation of the results of staining-; for it would 
seem that when (after using Heidenhain) we stain strongly 
with an acid stain, then in the resulting preparation the 
protoplasm masks the chromatin ; on the other hand, when 
(after Heidenhain) we stain weakly with an acid stain or do 
not use one at all, the chromatin masks the protoplasm. The 
chromosomes, centrioles and outer part of the karyosomes, 
since they consist entirely of chromatin, when once stained 
with Heidenhain keep their black appearance unaltered by 
any subsequent treatment with acid stains. 
Tn conclusion, I w'isli to express my thanks to Professor 
jMinchin for his friendly advice as to literature, and also for 
his criticism of this paper. 
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