PHYSIOLOGY OF LAMELLIBEANCH BLOOD-COEPUSCLES. 611 
Where the healing of a wound in later stages was followed, 
the animal was narcotised with the foot in an extended con- 
dition by a 1 per cent, solution of cocaine in sea-water; the 
required portion of the foot was then cut off, and fixed, and 
sectionised as before. In these experiments it is advisable 
to keep the animals in separate basins with a good flow of 
watei’, as if overcrowded, a number die, presumably from 
infection through the wound. 
Methods of Studying Phagocytosis. 
The bacteria used for this purpose were obtained by inocu- 
lating peptonised fish-broth, made with sea-water, with a 
platinum loop which had been passed along the edge of the 
mantle. This resulted in a mixed culture of bacteria, in 
which a rather large non-motile bacillus with rounded ends, 
and a tendency to form diplo-bacilli, much predominated. 
The actual process of phagocytosis was observed by adding 
a loopful of the diluted culture to a drop of blood. Permanent 
slides, showing ingested bacteria, were prepared by leaving a 
drop of blood, to which bacteria had been added, for from 
one to two hours in a moist chamber, and then fixing and 
staining by the same method as that employed in the prepara- 
tion of stained blood-films. 
Further observations were made by filling thin-walled 
capillary tubes with cultures of bacteria, sealing them at one 
end, and introducing the open end into a drop of blood on a 
slide. A cover-glass with a small quantity of wax at each 
corner was then placed on the drop, and the tube containing 
the bacteria kept under observation. 
Similar experiments were conducted by introducing capillary 
tubes containing cultures, etc., into the adductor inuscle of 
the animal, which may be replaced in the water and left for 
some hours. The tubes were then withdrawn, and the number 
of corpuscles which had entered the tubes were noted under 
the microscope. 
In these experiments the capillary tubes should be marked 
VOL. 54, PART 4. NEW SERIES. 43 
