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act as reservoirs. Virus isolations will be attempted from islanders who 
are ill with apparent and inapparent virus-induced illnesses, as well as 
from arthropod and ferral animals. Attempts will be made to isolate 
cytomegalovirus from urines of children during pre- and post-natal periods. 
Detailed medical examinations will be conducted to elicit long-term 
neurological sequellae in humans having a history and serological evidence 
of previous acute viral infections. 
Fibroblast Collections: Human fibroblast will be obtained from as many 
people as possible by skin biopsy. The specimen will be maintained in 
tissue culture on shipboard as monolayer cultures of cells. Cells in 
culture will be frozen in cryoprotective medium in liquid nitrogen 
according to techniques developed in this laboratory for field useage. 
Upon return to the NIH, cells will be revived for a wide range of studies, 
including cytology identification of polymorphisms and attempts at virus 
Isolation. This project is intended to be a pilot study for population 
genetics of the future where fibroblasts will be revived for study of new 
genetic polymorphisms as techniques and knowledge evolves without depleting 
a stock of biological material, as now happens with serum and red cells 
collected on a one-time only basis. 
Funding, Travel, Supplies and Equipment: A large amount of equipment, such 
as small boats, engines, generators, extensive laboratory and medical 
supplies and equipment are being supplied and paid for with NSF grant funds 
held by the Scripps Institute. Additional equipment necessary to support 
most phases of our work is being provided by Dr. Damon from expedition "A". 
Twenty thousand dollars of NSF funds are being provided for travel and 
other expenses of participants. Funds have been allocated also for 
shipment of medical research supplies in the amount of $2500. Scripps has 
already expended a further $4000 for equipment and supplies not carried in 
their inventory, $1500 for a small ultralow termperature freezer, and an 
additional $3400 for travel and per diem expenses of local officials whose 
participation in this type of expedition is essential. 
Additional supplies are being provided from our own laboratories by 
utilization of stock supplies, equipment on hand, supplies diverted from 
New Guinea field studies and supplies purchased for unique phases of this 
particular type of field study, including drugs for emergency treatment of 
personnel and islanders. X-ray film and developers, photographic material, 
tissue culture supplies, clinical and medical laboratory supplies, bleeding 
venules and needles, etc. have been collected at NIH and are awaiting 
approval for shipment. NIAID Reference Reagent Branch has provided all 
antigens for use in seroepidemiological surveys, at no expense to NINDS. 
Portable x-ray equipment is being left on board by Dr. Damon for our use. 
A liquid nitrogen freezing unit, purchased for use in our Building 8 
laboratories, will be used on board ship and returned to our laboratory 
upon completion of this field trip. Cinema and still film used extensively 
in our on-going laboratory and field studies will be used to document 
clinical and anthropological findings. 
It is respectfully requested that approval be given for this project and 
that authorization be issued allowing for the immediate shipment of 
supplies from the NIH to Hawaii to insure arrival prior to July 23, 1972. 
