TABLE OF CONTENTS Page 
PART ONE: ’FINAL ENVIRONMENTAL IMPACT STATEMENT 
I. Foreword 1 
II. Authority 7 
III. Objective of the NIH Action 8 
IV. Background 9 
A. Description of the Recombinant DNA 
Experimental Process 9 
B. Events Leading to the Development of Guidelines .... 15 
C. Description of Issues Raised by Recombinant DNA 
Research 2 3 
1. Possible Hazardous Situations 2 3 
2. Expected Benefits of Recombinant DNA Research. . 31 
3. Long-Range Implications 3 6 
4. Possible Deliberate Misuse 38 
V. Description of the Action 44 
A. General Application of the Guidelines 44 
B. Physical Containment Requirements 47 
C. Biological Containment Requirements 53 
D. Combination of Physical and Biological 
Containment in Practice 55 
E. Compliance with the Guidelines 62 
VI. Description of Alternatives 65 
A. No Action 65 
B. NIH Prohibition of Funding of All Experiments 
with Recombinant DNA 66 
C. Development of Different Guidelines 67 
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