19 13- L 4.] Enzymatic Peptolysis in Germinating Seeds. 115 
being formed after a period of fourteen days in the Extract B digestions at 
15°. The remainder of the digestions gave negative results after three weeks’ 
incubation, when the experiment was discontinued and the Pepton Witte 
digestions precipitated. The filtrates from these were used in the manner 
described by Weis (l.c.) for determining the degree of peptolysis which 
had taken place during incubation, expressed in terms of cubic centimetres 
of N/10 alkali, this latter figure representing the ammonia formed during 
the determination of the nitrogen contained in 5 c.c. of the filtrate, by 
Kjeldahl’s method. 
The results of the experiment may be seen in the following tables 
Extract A, with 
1. Pepton Witte. 
Temperature 
of 
Digestion. 
Titrations. 
Average. 
Control. 
Difference 
(indicating enzyme 
action). 
15° 
1. 2. 
8*9, 9T, 
3. 
9*5 
9T6 
14*9 
5-74 
25° 
6-0, 5-5, 
5-6 
5 - 7 
15-3 
9-6 
37° 
6T, 6’1, 
— 
6T 
15*3 
9*2 
50° 
8-3, 8-4, 
— 
8*35 
15-5 
7'15 
2. Pepton Roche. 
The whole of this series of digestions gave negative results. 
Extract B, with 
1. Pepton Witte. 
Temperature 
Difference 
of 
Titrations. 
Average. 
Control. 
(indicating enzyme 
Digestion. 
action). 
1. 2. 3. 
15° 
13*25, 12-55, 12-55 
12-78 
15-8 
3-0 
25° 
7-7, 7-4, 7-1 
7-4 
16-8 
9-4 
37° 
7-2, 8-2, 6-65 
7-35 
16-85 
95 
50° 
10-15, 9-0, 
9-55 
16-0 
6-45 
2. 
Pepton Roche. 
15°. Positive result observed after fourteen days. 
25°. Strongly positive result within six days. 
37°. Positive result also within six days. 
50°. Negative result. 
