1913-14.] Enzymatic Peptolysis in Germinating Seeds. 121 
so is present in the cells of the fruit, and might be extracted by the 
Buchner method, was not determined at the time. 
Juice of An an ass a sativa on 
1. Protein. 
Reaction of 
Medium. 
Titrations, 
[c.c. N/10 NaOH] 
Average. 
Control. 
Difference 
(indicating 
enzyme 
action). 
Acid 
2-5, 2-55, — 
2*52 
8-5 
60 
Neutral .... 
6*6, 7-0, 57 
6'46 
8T 
1-64 
Alkaline .... 
6-8, 6-6, 5*7 
6-4 
8-6 
2-2 
2. Pepton Witte. 
Acid ..... 
2*4, 2'4, — 
2-4 
5-6 
32 
Neutral .... 
4-4, 4-1, — 
4*25 
5-9 
1-65 
Alkaline .... 
5-2, 5-5, 6-0 
5-56 
6-0 
•44 
3. Pepton Roche. 
The whole of this series gave negative results. 
About the same time a number of fungi were gathered, ground to pulp 
with sand in a mortar, and the juice pressed out in the hand -press. The 
preparations thus obtained were used in a similar series of experiments, 
the only difference being that the digestions were in this case confined to 
the natural reaction of the extracts, in all cases slightly acid. 
The results were as follows 
Fungus. 
Protein Digestions. 
Pepton Witte Digestions. 
Titrations. 
Aver- 
age. 
Con- 
trol. 
Diff. 
Titrations. 
Aver- 
age. 
1 n 
Con- 
trol. 
Diff. 
Lycoperdon gemmatum 
9-2, 
9-35, 
9T5 
9-23 
9-45 
•22 
5*6, 5-3, 5-2 
5-36 
8-4 
3*0 
Hyphaloma capnoides . 
8-75, 
8-95, 
8-85 
8-85 
9-5 
•65 
5-8, 5-9, 5-7 
5-8 
8-5 
2-7 
Hyphaloma trichaloma 
9-15, 
9-0, 
9-2 
9:12 
9-4 
•28 
6-1, 61, 6T 
6T 
8-4 
23 
Russula emetica . 
9'1, 
9-2, 
9T 
9T3 
9*3 
T7 
6-8, 6-7 — 
6-75 
8-6 
1*85 
Boletus badens 
8-9, 
8*7, 
8-9 
8'83 
93 
•5 
5 3, 5*5, 5-4 
5*4 
8-3 
2-9 
Laccaria laccata . 
10*0, 
9-85, 
9-6 
9-8 
9-85 
•05 
6-9, 6*7, 7-0 
6-86 
8-8 
1-94 
Hydnum repandum 
.9-3, 
9*1, 
9-2 
9-2 
93 
T 
7-0, 6-6, 6-4 
6-6 
8-7 
2T 
Amanita rubescens 
7-0, 
7-6, 
7*5 
736 
8-9 
1-54 
3-9, 4-0, 4T5 
4-0 
7-7 
3*7 
Amentopsis strangulata 
7*7, 
7-65 
8-0 
7-78 
8-9 
1-12 
4-7, 4-7, 4-5 
4-63 
7‘8 
317 
Pepton Roche. 
The whole of these gave negative results. 
