124 Proceedings of the Royal Society of Edinburgh. [Sess. 
tion, and the nitrogen content determined in the usual way. The results 
were as follows : — 
Titrations. 
Average. 
Control. 
Difference. 
Protein .... 
87, 8*6, 8-5 
86 
9*3, 9T 
0-6 
Pepton Witte 
4T, 4T5, 4-2 
4-15 
8-0, 8-2 
3-95 
III. A watery extract was first obtained, as in the first experiment, and 
the residue subjected to the Buchner method process in order to obtain the 
cell contents. The same conditions were observed as in the previous experi- 
ments, with the difference that the digestions were carried out with Pepton 
Witte and Pepton Roche only and that four temperatures were employed, 
viz. 15°, 25°, 37°, 50°. 
The results will be seen from the following table : — 
Extract A, with Pepton 
Witte. 
Temperature. 
Titrations. 
Average. 
Control. 
Difference. 
15° 
7-9, 7-4, 8-2 
7-83 
7 '9 
•07 
25° 
8-3, 8-5, 7-9 
8*23 
8-5 
•27 
37° 
8-0, 8-0, 8-0 
8-00 
8-0 
•00 
50° 
7-9, 8-0, 8-0 
7-97 
8T 
T3 
Extract A, with Pepton 
Boche. 
All results were negative. 
Extract B, with Pepton Witte. 
15° 
6-0, 6*4, 5-9 
6T0 
7-60 
1-50 
25° 
2-35, 2-5, 2-8 
2-55 
7-60 
5 05 
37° 
2-4, 2-0, 
2-20 
7-50 
5*30 
50° 
3-6, 3-6, 33 
3-76 
7*40 
394 
Extract B, with Pepton 
Roche. 
15°, 
. Slight positive result within twenty-four hours. 
25°. 
Strong 
55 
55 
37°. 
55 55 
55 
>5 
50°. 
Negative result at the end of three weeks. 
In the case of Penicillium glaucum and Aspergillus niger, pure cultures 
were developed in sterilised 10 per cent, malt extract in large flasks at 
room temperature. After several weeks a large growth had taken place, 
