125 
1913-14.] Enzymatic Peptolysis in Germinating Seeds. 
and all the cultures were in a state of fructification. As much as possible 
of the liquid was then poured off and discarded, while the liquid remaining 
amongst the mycelium was expressed by means of the hand-press. This 
was retained for experiment as Extract A. 
The mycelium was then ground in the usual way in the Buchner 
mortar and pressed in the Buchner press. In this way two liquids were 
obtained from each of the fungi — a medium, and a mycelium extract- 
Digestions were made as before with these preparations, using protein, 
Pepton Witte, and Pepton Roche as substrates. The results may be seen 
in the following tables : — 
Penicillium glaucum — Extract A (Hand-press Extract) on 
Titrations. 
Average. 
Control. 
Difference. 
1. Protein .... 
8-3, 8T5, 8T5 
8-2 
9-45 
1-25 
2. Pepton Witte 
8-4, 8-7, 8-2 
8-46 
8-9 
•44 
3. Pepton Roche 
All negative. 
Extract B (Buchner Extract) on 
1. Protein .... 
6-4, 7*0, 6-7 
6-7 
8-8 
2*1 
2. Pepton Witte 
4-8, 5*0, 5-2 
5-0 
8-6 
3-6 
3. Pepton Roche 
All negative. 
Aspergillus niger — Extract A on 
Titrations. 
Average. 
Control. 
Difference. 
1. Protein .... 
8-0, 7*0, 7-5 
7-5 
9*3 
1-8 
2. Pepton Witte 
6*2, 6*1, 6-0 
6T 
8-9 
2-8 
3. Pepton Roche 
Negative result. 
Extract B on 
1. Protein . 
6-75, 6-9, 6-3 
6-65 
8-3 
D65 
2. Pepton Witte 
2-2, 2-5, — 
2-35 
6-8 
4-45 
3. Pepton Roche 
In the apparently complete absence of the Pepton Roche hydrolysing 
activity in Penicillium glaucum, a striking contrast is shown with Asper- 
gillus. In view of the close relationship existing between these fungi, it 
was thought desirable to ascertain if Penicillium could be brought to 
