MEDICAL RECORD 
STUDY NUMBER: 
CONTINUATION: J^pagts. 
examine the lymphocytes that you are to be given to decrease the chance of 
this occurrence. 
Although administration of immune lymphocytes can shrink tumors in some 
patients, many patients do not respond to the procedure and even those that 
exhibit shrinkage may show regrowth of the tumor after a short period of time 
(months ) . 
Tumor Necrosis Factor 
Recent scientific advances have made it possible to modify the genetic 
makeup of tumors so that they can stimulate a greater response by the immune 
system. The scientific advance is called "retroviral-mediated gene transfer" 
and allows us to insert a gene into some of your tumor cells, stimulating them 
to produce a hormone called tumor necrosis factor (TNF) . This hormone is 
naturally produced in the body in small amounts. The cells we give you may, 
however, produce too much TNF throughout the body. TNF is thought to play a 
role in body defenses against cancer. Giving too much TNF may be dangerous 
and may cause life-threatening low blood pressure. TNF can also cause fever, 
chills, low urine output, weight loss, nausea, vomiting and fatigue. We have 
inserted into your tumor, by a special laboratory technique, not only the 
human gene for TNF but a second gene taken from a bacterium that causes the 
tumor cells to be resistant to an antibiotic, neomycin. A gene is a part of a 
chromosome (hereditary material) that contains the information a cell needs to 
make proteins. 
The following procedure was used to insert these genes into your tumor. 
The tumor cells were attached to mouse virus genetic material that has the 
Recombinant DNA Research, Volume 15 
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