Page No. 
A Description of Proposal 
68-76 
B. Special Issues 
76 
C. Requested Documentation 
76-77 
1 . Curriculum Vitae for key personnel 
78-138 
Submission to IRB 
138-147 
Appendix 
A. Wilson JM, Jefferson DM, Chowdhury JR, Novikoff PM, Johnston DE and 
Mulligan RC. Retrovirus-mediated transduction of adult hepatocytes. Proc. Natl. 
Acad. Sci. USA, May 1988; 85:3014-3018. 
B. Wilson JM and Chowdhury JR. Prospects for gene therapy of familial 
hypercholesterolemia. Mol. Biol. Med., 1990; 7:223-232. 
C. Grossman M and Wilson JM. Genetic intervention in the prevention of coronary 
heart disease, in press. 
D. Wilson JM, Johnston DE, Jefferson DM, and Mulligan, RC. Correction of the 
genetic defect in hepatocytes from the Watanabe heritable hyperlipidemic rabbit. 
Proc. Natl. Acad. Sci. USA, June 1988; 85:4421-4425. 
E Wilson JM, Chowdhury NR, Grossman M, Gupta SJ, Jeffers J, Huang T-J, and 
Chowdhury JR. Transplantation of allogeneic hepatocytes into LDL receptor 
deficient rabbits leads to transient improvement in hypercholesterolemia. Clin. 
Biotech., Spring 1991 ;1-6. 
F. Wilson JM, Chowdhury NR, Grossman M, Wajsman R, Epstein A, Mulligan, RC, 
and Chowdhury JR. Temporary amelioration of hyperlipidemia in low density 
lipoprotein receptor-deficient rabbits transplanted with genetically modified 
hepatocytes. Proc. Natl. Acad. Sci. USA, Nov 1990; 87:8437-8441. 
G Chowdhury JR, Grossman M, Gupta SJ, Chowdhury NR, Baker JR, and Wilson JM. 
Ex vivo gene therapy in LDL receptor deficient rabbits leads to permanent 
improvement of hypercholesterolemia. Submitted 
H. Grossman M, Raper S, and Wilson JM. Towards liver-directed gene therapy: 
Retrovirus mediated gene transfer into human hepatocytes. In press. Somatic 
Cell and Molecular Genetics, 1991. 
I. Danos O and Mulligan RC. Safe and efficient generation of recombinant 
retroviruses with amphotropic and ecotropic gene transfer. Proc Natl Acad Sci 
USA, Sep 1 988;85(1 7):6460-4. 
J . Components of media. 
Recombinant DNA Research, Volume 15 
