13.0 Preliminary Data 
I. T cell dependence and specificity. 
Recently, we have obtained data that T cells are required 
for tumor rejection in vivo. Injection of the MCA 106 
fibrosarcoma with /9-galactosidase (n=5) , or MoLV LTR H2-K 3 
plasmid (n=5) resulted in no tumor protection. Tumor growth was 
comparable to uninjected tumors in nude mice (data not shown) . 
A. B. 
WEEKS 
Figure A. Lack of tumor protection following introduction of H- 
2K S into tumors in nude mice. BALB/c nu/nu mice were inoculated 
with (A.) CT26 or (B.) MCA 106 tumors and injected with the H-2K S 
(■) or /5-galactosidase (□) expression vector at the indicated 
times (see arrow). Results represent average tumor size of each 
experimental group (n=5) . 
In addition, the deletion of the al and q 2 domains from the 
H-2K S expression vector results in a loss of protection. 
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Recombinant DNA Research, Volume 15 
