In both experiments, tumors transfected with the H-2K S gene 
stimulated pre-effector cell responses in the draining LN from 
which immune effector cells were generated by the anti-CD3/IL-2 
activation. There was a significant reduction in the numbers of 
pulmonary metastases in animals treated with these cells. These 
results demonstrate that in vivo gene transfer of a poorly 
immunogenic mouse melanoma with DNA encoding allogeneic MHC 
determinants enhances the host T cell response to these tumors. 
Since the time of presentation of this data at the November 21 
meeting, this experiment has been repeated an additional two 
times, with similar results. 
VI. Induction of immune response to HLA-B7 in large animal 
model. 
The three vectors described in the proposal have been tested 
for their ability to stimulate vasculitis in vivo in porcine 
arteries. A focal vasculitis has been induced in porcine 
iliofemoral arteries two weeks after introduction of each of 
these vectors by DNA liposomal transfection. Each of these 
vectors successfully conferred tumor regression when used in the 
mouse model with H-2K S , as described in the original proposal 
with the retroviral vector. These data further support the 
observation that direct introduction of a foreign 
histocompatibility gene confers both local and systemic 
protective effects against tumor cells in vivo and may be useful 
in the treatment of human malignancy. 
Recombinant DNA Research, Volume 15 
[435] 
