6.213) 
Post-transduction marrow (prior 
to freezing) to assess LNL-6 
treated cells immediately after 
transduction 
a. Bone marrow cells at 
harvest (treated) 
4 x 1 0 7 cells in 
preservative-free heparin 
analysis: see 6.212 above 
6.214) Post-transplant samples to be 
collected 
day 21 
ANC >1500 and platelets >50,000 
untransfused 
day 100 
yearly 
a. Bone marrow aspirate and 
biopsy 
light microscopy 
(cytogenetics day 100) 
b. Bone marrow cells 
5 ml of marrow 
analysis: 
2xl0 6 cells for neo R PCR 
lxlO 7 cells for Southern 
blot analysis 
2 x 1 0 6 cells for 
methylcellulose assay 
c. Blood - cells 
10-20 ml in preservative- 
free heparin 
analysis: see 6.214b 
above 
d. Blood - serum (collect 
day 100 and 1 year) 
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Recombinant DNA Research, Volume 15 
