1.0 CHECKLIST FOR PATIENT ELIGIBILITY PRIOR TO BMT 
NO YES 
Age less than 21 years at time of diagnosis 
Receiving matched unrelated donor or mismatched family member 
BMT for leukemia on or as per the MISMUD protocol 
CHECKLIST FOR ELIGIBILITY AT TIME OF ADMINISTERING EBV 
SPECIFIC CTLs 
Cytotoxic T cell line availability (Call Ext 2529 or 375) 
No GVHD or Grade I GVHD 
ANC > 500/mm 3 
Bilirubin <2 x normal, SGOT <3 normal; prothrombin time normal 
Creatinine < 2 times normal for age or creatinine clearance > 2 times 
normal for age 
Normal electrolytes, calcium, phosphorus, nutritional status 
No severe intercurrent infection 
Life expectancy of at least 6 weeks and Performance status (ECOG) 
of 0-2. 
To check eligibility or register patient call Dr. Heslop at 531-2529 or Dr. Brenner at 
522-0410. 
Informed consent explained to and signed by patient/guardian 
Check table in Section 7.2 of this protocol to see necessary baseline 
and follow-up studies 
2.0 OBJECTIVES 
2.1 To generate EBV specific cytotoxic T cell lines from BMT donors 
2.2 To determine the safety of four weekly intravenous injections of BMT donor 
derived EBV specific cytotoxic T lymphocytes marked with the neomycin 
resistance gene in patients transplanted with marrow from a matched 
unrelated or mismatched family member for leukemia. 
2.2 To determine the survival and immunologic efficacy of neomycin gene marked 
EBV specific cytotoxic T lymphocyte lines in bone marrow transplant 
recipients. 
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Recombinant DNA Research, Volume 17 
