hydroperoxycyclophosphamide for ex vivo use. Pergamid™ is soluble in water, 
saline, or tissue culture medium. 
9.4.1 Indication 
Pergamid™ is indicated for the ex vivo pharmacologic purging of 
autologous bone marrow of residual neoplastic cells. 
9.4.2 Dosage and Use 
Bone Marrow Purging : The autologous bone marrow sample should be 
treated with Pergamid™ at a final concentration of 60 /ig/ml. 
9.4.3 Preparation and Handling of Solutions 
Pergamid™ should be stored at or below -20°C in a desiccator. The 
desiccator should be at room temperature for one-half hour prior to 
opening. 
Pergamid™ should be reconstituted by adding 20 ml sterile saline or 
tissue culture medium to the 50 ml vial and shaking until all 
particulate material has disappeared. This results in a concentration 
of 10 mg Pergamid™ per ml of solution in the vial. For details of 
marrow treatment see Appendix A. 
9.4.4 How Supplied 
Pergamid™ is supplied in sterile vials for single use ex vivo containing 
200 mg 4-hydroperoxycyclophosphamide. Storage at or below -20°Cis 
required. Pergamid™ solutions should be used within one hour of 
reconstitution. 
9.5 Interleukin-2 
9.5.1 Storage stability of nIL2 Biotest 
nIL2 Biotest can be stored for over a year at temperatures in the range 
from +2°Cto +8°C. From preliminary storage tests carried out at 
other temperatures, it can be assumed that the nIL2 preparation can 
be stored for up to 6 months at temperatures in the range from +15°C 
to +25 °C (room temperature). Storage at higher temperatures is 
inappropriate. 
9.5.2 Biotest nIL2 is provided in liquid form in ampoules of 1 ml (10 5 units). 
[250] 
Recombinant DNA Research, Volume 17 
