d. All patients will have central venous access, preferably a double 
lumen catheter system. 
e. VS flow sheet at the bedside 
f. Normal saline for IV infusion 
g. Baseline vital signs are taken and recorded. 
h. Patients receive apre-med with Benadryl and hydrocortisone 15 
minutes prior to infusion. 
11.5.3 Marrow Infusion 
a. After thawing rapidly in 37°C water bath, the marrow is 
administered by the physician as rapidly as possible. A 
physician and nurse both MUST remain in the room for the 
total infusion time and one or the other for- 1 hr post infusion. 
NOTE: The marrow MUST NOT be FILTERED . 
b. Vital signs are taken q 15 min. x 4; Q 30 min. x 4, Q hour x 4 
and recorded. Patient is monitored by oximeter during infusion 
and for 6 hours afterward. 
11.5.4 Toxicity of Infusion 
a. Volume overload: 
This is of particular importance in small recipients. 
b. Pulmonary' emboli 
Rare complication that might occur due to fat and marrow cell 
emboli. Marrow and fat emboli cause a transient alveolar 
capillary block and administration of 0 2 or IPPB may be neces- 
sary'. 
c. Allergic reactions 
Chills, fever and hives occasionally occur. These reactions are 
never severe and respond to additional parenteral diphen- 
hydramine (benadryl). 
Recombinant DNA Research, Volume 17 
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