i. Cytotoxic antibody screen 
j. Surveillance cultures 
k. HIV, CMV, hepatitis, A, B and C screens 
4. Bone marrow aspirate and biopsy 
5. Lumbar puncture for culture and cytology 
6. Bone marrow for karyotype analysis 
7. Skin test: multi-test unit (by IDS) 
8. 5 cc reference serum 
9. If available, bone marrow cells and peripheral blood leukocytes will be 
stored for future studies. 
13.2 During and Post-Transplantation (Months 0-3) 
1. A daily CBC, differential and platelet estimate until self-sustaining and 
then as indicated. 
2. Daily electrolytes during cytoxan and at least weekly SGOT, SCiP” 
total bilirubin, alkaline phosphatase for the first 45 days. 
3. EKG on days -5, -4, -3, -2, before each dose of cytoxan, and again on 
days 1 and 7 post-marrow reinfusion. 
4. Viral, bacterial and fungal cultures as clinically indicated. 
5. Chest x-ray weekly for 3 weeks and as indicated. 
6. Bone marrow aspirate and biopsy days +21, +32 and day +100 post 
marrow reinfusion for morphology and as indicated, plus neo-gene 
content on colony culture. Lumbar puncture at the end of the third 
month. 
7. Patients with CNS involvement will receive 5 spinal taps with 
methotrexate (see Section IV. C. 2.) after hematopoietic recovery. 
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Recombinant DNA Research, Volume' 17 
