Table 11. Evaluation of the range of irradiation doses that 
result in 100% inhibition of proliferation of renal cell 
tumors in vitro. 5 fresh renal tumor cells of varying in 
vitro passages between 1 and 5, were irradiated with a range 
of doses from 5,000 to 40,000 rads and plated at a density of 
1 x 105 cells per 25 cm2 flask. Cells were scored daily for 
in vitro growth and cell death. All of the renal cell tumors 
were evaluated prior to transduction. 
TUMOR CODE POST- IRRADIATION 
EVENTS IN VITRO 
RANGE OF HISTOLOGY 
IRRADIATION 
DOSES 
RCC 7.18.91 
RCC 10.14.91 
RCC 11.4.91 
RCC 6.23.92 
RCC 6.29.92 
DAY 5 - Slow orowth 
all doses 
DAY 9 - 20% Cell death 
at >15,000 rads 
DAY 17 - 50% Cell 
death at all doses 
DAY 28 - 100% Cell 
death all doses 
5000-30000 rads 
Papillary, grade 
II 
DAY 3 - Enlaraed cells 
at >15,000 rads 
DAY 7 - Enlaraed 
cells, no growth all 
doses, 10% death 
>14,000 rads 
DAY 14 - 50% Cell 
death all doses 
DAY 27 - 100% Cell 
death at all doses 
5000 - 23000 
rads 
Sarcomatoid 
DAY 3 - No growth. 
12000 - 33000 
Grade II 
enlarging cells 
>26,000 rads 
DAY 4 - Slow arowth. 
enlarging cells > 
12,000 rads 
DAY 6 - 10% Cell death 
>12,000 rads 
DAY 19 - >90% Cell 
death at all doses 
rads 
Oncocytoma 
DAY 4 - >15% Cell 
death at all doses 
DAY 15 - >90% Cell 
death at all doses 
23000 - 39000 
rads 
Grade II, Cystic 
DAY 3 - >15% Cell 
23000 - 39000 
Clear cell. 
death at all doses 
DAY 13 _ >90% Cell 
death at all doses 
rads 
Grade II 
Recombinant DNA Research, Volume 17 
