a 
n 
A 
CN 
% 
a 
« 
o 
U3 
tu o 
01 H 
Rads (x 1000) 
0 Day 1 post Irradiation 
O U) O IO 
r r* 
Rads (x 1000) 
Day 4 post inradiation 
Figure 5. Evaluation of the effects of irradiation on GM-CSF 
production by transduced renal carcinoma cells. Transduced 
RCC vaccine cells were either irradiated with a total dose of 
5,000, 10,000 or 15,000 rads, or left untreated, and 
subsequently cultured for 4 days. Supernatants were 
collected every 24 hours and replaced with fresh tumor media. 
The 24 hour supernatants for days 1 and 4 were assayed for 
GM-CSF production by ELISA. The results revealed that 
irradiated, transduced cells produced at least as much GM-CSF 
as did the non-irradiated controls, demonstrating that the 
lymphokine secreting capacity of the vaccine cells was not 
impaired by radiation treatment. 
Recombinant DNA Research, Volume 17 
[307] 
