cells are observed for cytopathic effect for the entire 28 day period. Hemagglutination and 
hemadsorbtion are performed twice during the assay. 
In Vivo Vims Assay; M.A. Inc, Protocol No. 1514.005002: This test includes the inocula- 
tion of adult mice, suckling mice, guinea pigs and embryonated hens eggs via yolk sack and 
allantoic routes of inoculation. All animals are observed for clinical signs of viral infection. 
Suckling mice are sacrificed on day 14 for the preparation of a tissue homogenate which is 
inoculated into a new group of suckling mice. Specific Pathogen Free (SPF) eggs are examined 
for viability and the blind passage of fluids into new egges is performed. 
Replication Competent-Adenovirus Assay; M.A. Inc, Protocol No. 1514.031002: This 
assay is designed to detect the presence of human adenovirus in the test article. Indicator cell 
cultures are inoculated and observed periodically for cytopathic effect (CPE), a passage is 
performed for enhancement. 
A deno- Associated Vims Hybridization; M.A. Inc, Protocol No. 1516.104017: This assay 
detects adeno-associated virus DNA present in the test article as determined by Southern 
hybridization using a 32 P-labelled DNA probe. 
HIV Co-cultivation Assay; M.A. Inc, Protocol No. P1516.015001: This procedure is 
designed to detect small amounts of retrovirus that may be present in the test article. To amplify 
any virus present, selected Peripheral Blood Lymphocytes (PBL) cells are infected, mixed with 
test article, passaged and analyzed for HIV by observing for cytopathic effect (CPE) and 
production of viral antigens by antigen capture ELISA. 
Hepatitis B; M.A. Inc, Protocol No. P1516.040: This procedure is designed to detect 
small amounts of Hepatitis B surface antigen that may be present in the test article which would 
be detected by a third generation ELISA assay. 
Cytomegalovirus; M.A. Inc, Protocol No. P1514.030: The test and control articles are 
directly inoculated onto cell cultures (indicator cells) and examined for 42 days for the 
appearance of cytopathic effect. Additionally, cells are fixed and examined using immunoflu- 
orescent techniques. 
Bovine Virus Assay; M.A. Inc, Protocol No. 1514.032001: This study is designed to 
detect bovine viruses such a$ Bovine Viral Diarrhea Virus (BVD), Infectious Bovine 
Rhinotracheitis Virus (IBR), Parainfluenza 3 (PI 3), Bovine Adenovirus (BA), or Parvovirus 
(BP) using sensitive indicator cells and immunofluorescence and cytopathic effect (CPE) as 
endpoints. 
Porcine Parvovirus; M.A. Inc, Protocol No. P1514.033004: This assay is designed to 
detect Porcine Parvovirus (PPV) in cell cultures. Test and control articles are analyzed by 
inoculation of indicator cell cultures and examined for at least 14 days for the presence of CPE. 
Cultures are also tested for the presence of specific viral antigens using fluorescent antibody 
techniques. 
Parvovirus B-19 Hybridization; M.A. Inc, Protocol No 1516.104017: This protocol is 
designed to detect parvovirus B-19 DNA present in the test article as determined by Southern 
hybridization using a 32 P-labelled DNA probe. 
Epstein Bar Vims (EBV) Probe Assay; M.A. Inc, Protocol No. P1516.104: The purpose 
of this study is to detect EBV DNA that may be present in the test article as determined by 
Southern hybridization using a labeled DNA probe. DNA is extracted from the test article cell 
pellet and blotted onto membranes. Blotted membranes are hybridized to a labeled EBV probe 
and detected by autoradiography. DNA from Namalwa cells are included as a positive control. 
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Recombinant DNA Research, Volume 17 
