riboprobes under conditions specific for detection hCFTR mRNA. Similar studies, (not shown) 
demonstrated j3-galactosidase mRNA directed by AvlLacZ4 (6 x 10 8 pfu) and X-gal staining 
demonstrating synthesis of bacterial /3-galactosidase by the infected cells of the cotton rat. In 
these animals, dosed intranasally by aspiration, viral infection was not homogenous, being 
confined to specific lung segments, other segments being entirely free of hCFTR mRNA. 
Immunohistochemical staining of these sections is in progress at the time of this submission. 
Summary of Functional Data From AvlCF2 Adenovirus: AvlCF2 virus directed the 
synthesis of the human CFTR mRNA producing the hCFTR polypeptide that conferred cAMP 
dependent changes in conductance, intracellular Cl' and Cl' efflux in response to forskolin. 
These characteristics are consistent with the appropriate function of the transferred hCFTR 
polypeptide to the CFPAC-1 cells by the AvlCF2 vector. 
Recombinant DNA Research, Volume 17 
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