One week later (day 7) and two weeks later (day 14) the history and physical will be 
repeated, the chest x-rays will be repeated together with oxygen saturation tests and 
pulmonary function tests. The lung scans will be repeated 7 days later. Virus cultures 
will be obtained and blood tests will be collected, as well as urine tests. If I am well and 
if the cultures from my throat swabs and stool show that no adenovirus is being excreted 
from my body at day 14, 1 will be discharged. In the unlikely event that I am still 
excreting the adenovirus construct, I will be asked to remain in isolation in the clinical 
research center until the virus has been eliminated. 
I will then be followed in the outpatient clinic, and every week I will attend for a history 
and physical, oxygen saturation tests, pulmonary function tests, adenovirus cultures, blood 
tests, and urine tests. The chest x-ray will be performed again on day 28. During this 
period of time when I am at home following gene therapy, I will be asked to avoid 
contact with people with known infections and young children, and to avoid crowded 
places such as movie theaters and shopping malls. 
On day 55 I will be readmitted to the clinical research center and will have a history and 
physical, chest x-ray, lung scans, oxygen saturation tests, pulmonary function tests, virus 
cultures, blood tests, urine tests, sputum culture, and if I am female, a repeat pregnancy 
test. 
On the following day (day 56) the voltages in my nose will be measured again. After 
anesthetizing the nose with lidocaine, the nose will be brushed and small biopsies taken. 
I will then have my upper airways anesthetized with lidocaine, and with intravenous 
sedation I will have a repeat bronchoscopy with bronchial washings, brushings, and small 
biopsies. After collecting tissues the doctors will administer a dose of the adenovirus- 
CFTR construct directly into one selected lobe, which will be the same lobe that was 
treated before. At the end of the procedure, the doctors will "paint" the inside of my 
windpipe with the virus suspension again. Adenovirus cultures will be collected prior to 
bronchoscopy and administration of the construct. Blood tests and a urine test will be 
performed. 
On day 59 a history and physical, chest x-ray, oxygen saturation tests, pulmonary function 
tests, adenovirus culture from pharyngeal swabs and stool, blood tests, and urinalysis will 
be performed. The bronchoscopy will be repeated with intravenous sedation as before. 
The voltage inside my windpipe will be measured. Bronchial washings, brushings, and 
small biopsies will be obtained. 
On day 63 I will have a history and physical, chest x-ray, lung scans, oxygen saturation 
tests, pulmonary function tests, adenovirus cultures from pharyngeal swabs and stool, 
blood tests, and urinalysis. 
One week later (day 70) I will have the same tests, except for lung scans, which will not 
be performed. If my culture for the recombinant adenovirus is negative, I will come out 
of isolation and be discharged again. I will then return at weekly intervals for a history 
Recombinant DNA Research, Volume 17 
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