IVA*2. Recombinant ElA-deleted adenovirus: 
IVA-2.a. Expression of reporter gene (lacZ) in vitro 
IVA2.a.(i) Cultured airway epithelial cells: 
For these studies, an El-deleted adenovirus containing the lacZ marker gene 
under the control of the CMV promoter (Ad5-CMV-lacZ) was utilized. 
The ability of this El-deleted recombinant adenovirus to infect human 
airway epithelial cells was tested. Nasal turbinates were resected from normal 
donors and disaggregated epithelial cells cultured on tissue culture plastic in serum- 
free media. The cells were exposed to increasing concentrations of Ad5-CMV-lacZ 
(10^ to lCr pfu/ml) for 30 min, and the cells stained 2 days later for lacZ 
expression. This relationship is shown in Figure 3. 
[454] 
Recombinant DNA Research, Volume 17 
