These data show that ~ 10^ pfu/ml is a highly effective concentration. No 
evidence of cell toxicity was noted at this or higher concentrations for the 30 minute 
exposure regimens. 
Because of the availability of the CF mouse model (83,84), studies have been 
initiated to ascertain whether the Ad5-CMV-lacZ will infect mouse airway epithelial 
cells. In studies parallel to those outlined above, cultured nasal epithelial cells were 
exposed to recombinant adenoviruses for 30 min, and cell stained for lacZ 
expression. Dose-effect relationships are shown in Figure 4. 
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Recombinant DNA Research, Volume 17 
