Xenografts infected with the AdEldelta demonstrated no X-gal positive cells 
when harvested 3 days after exposure to vims (Figure 5D, inset), while large areas 
of lacZ expression were demonstrated in grafts exposed to Ad5-RSV-lacZ at 10 12 
pfu/ml and harvested at day 3 (Figure 5). Morphometric analysis of GMA sections 
of this xenograft indicated gene transfer in 11% of the epithelial cells (see Figure 
6 A and 6B below). 
A 
Localization of X-gal in various cell types of the epithelium. GMA sections (4 fJ. m) of xenografts 
infected with 10 iZ pfu/ml Ad5-RSV-lacZ adenovirus and harvested 3 days postinfection: (A), bar 
= 300 /im; and (B), bar = 50 /im. C, ciliated cells; G, goblet cells; B, basal cell, and I, intermediate 
cell. Arrow points to an aggregate of lacZ positive cells. 
Figure 6. Ad5-CMV-lacZ gene transfer in xenografts. 
Xenografts infected with Ad5-RSV-lacZ at 10^ and 10^ pfu/ml 
demonstrated gene expression in 1.9% and <0.1% of the total cells of the 
epithelium, respectively, when harvested at three days. 
Enface micrographs of these xenografts are provided in Figure 5 A (10^ 
pfu/ml) and Figure 5B (10^ pfu/ml). to analyze the stability of adenoviral lacZ 
expression within the bronchial epithelium, xenografts were also harvested 21 days 
following infection with lCr pfu/ml Ad5-RSV-lacZ adenovirus (Figure 5C). No 
changes in the percentage of X-gal positive cells were seen between 3 days and 21 
days postinfection (compare Figure 5B with 5C, respectively). 
Recombinant DNA Research, Volume 17 
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