IV.A.2.a.(iii) In vivo gene transfer in mice 
Direct administration of ElA-deleted recombinant adenovirus (Ad5-CMV- 
lacZ) has been performed in the nasal cavity of mice. Patchy expression in the nasal 
cavity was routinely seen (n = 9) with administration of 30 fi\ of 10** pfu/ml material 
into a single nostril (see Figure 10). 
A B C 
Figure 10. Epithelia excised from nasal cavity of mice. A: exposed to vehicle 
(100X);B: 3 days after exposure to Ad5-CMV-lacZ (100X); C: 3 days after 
exposure to Ad>CMV-lacZ (400X). 
Because of the irregularity of the nasal surface area, studies were performed 
in the trachea where the surface area is better defined for histologic analysis. As 
shown in Figure 11 below, administration of 30 ii\ of 10** pfu/ml yields more than 
50% expression of lacZ in all cell types of the trachea. 
[464] 
Recombinant DNA Research, Volume 17 
