IV.A.2.b.(iii) Adenoviral transduced CFTR and correction - 
transformed epithelial cells: 
The function of Ad5-CB-CFTR was evaluated in the cell line CFPAC which 
was derived from the pancreatic adenocarcinoma of a CF patient. Infectivity of the 
cell line with recombinant Ad5 was determined initially with lacZ adenovirus and 
subsequently with Ad5-CB-CFTR. CFPAC populations exposed to adenovirus at an 
MOI of 1 demonstrated expression in 30-40% of the cells. Recombinant CFTR 
protein expression was detected by immunocytochemistry in 100% of the cells after 
exposure to Ad5-CB-CFTR at an MOI = 500 (see Figure 13). 
PANEL A PANEL B 
CFPAC-l cells were exposed to Ad5-CMV-lacZ (panel A) or Ad5-CB-CFTR (panel B) at a MOI 
equal to 500. Three days later the cells were fixed in methanol and analyzed by 
immunofluorescence using an antibody to the COOH terminus of CFTR as described. 
Figure 13. Expression of CFTR by Ad5-CB-CFTR in CFPAC cells. 
The function of adenoviral-transduced CFTR protein was assessed by 
measuring cAMP mediated stimulation of Cl conductance using the halide sensitive 
fluorophone SPQ. In this assay, halide efflux is measured by an increase in 
fluorescence in cells loaded with SPQ and iodide. Results from mock infected 
CFPAC and CFPAC cells infected at an MOI of 500 with Ad5-CB-CFTR are shown 
in Figure 14. 
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Recombinant DNA Research, Volume 17 
