150 
3 135 
8 
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" 105 
n 
90 
I 
n 75 
l 60 
n 45 
s 
i 30 
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IS 15 
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0 200 400 600 800 1000 1200 1400 1G00 1000 2000 0 200 400 600 800 1000 1200 1400 1600 1800 2000 
I SECONDS ' SECONDS 
I 
Analysis of CFPAC cells for cAMP stimulated Cl* transport. CFPAC cells were exposed to Ad5- 
CB-CFTR (right panel) or Ad5-CMV-lacZ (left panel) and subsequently evaluated using the SPQ 
analysis. Details of this assay are provided in the text. 
Figure 14. Cl" transport in CFTR transduced CFPAC cells. 
Substitution of NO 3 with iodide in the medium of each population of cells 
produced little change in fluorescence. Subsequent increase in the intracellular 
cAMP by the addition of forskolin and IBMX stimulated a rapid rise in 100% of the 
cells infected the wild type Ad5-CB-CFTR virus which was quenched when iodide 
was substituted for NO 3 . None of the cells that were mock infected or infected 
demonstrated cAMP mediated changes in fluorescence. Cells infected with Ad5- 
CB-CFTR at MOFs ranging from 50 to 500 consistently produced good SPQ 
responses in 100 % of the cells. 
Recombinant DNA Research, Volume 17 
[469] 
