IVA.2.b.(iv) Correction of differentiated airway epithelia in 
primary culture from CF mice and human patients with 
cystic fibrosis: 
Primary cultures of both nasal and tracheal epithelia from CF mice have 
been established on collagen matrices that allow differentiation of ion transport 
capacity. Preparations from both nasal and tracheal regions of CF mice have been 
exposed to Ad5-CB-CFTR (lO 1 ^ pfu/ml x 24 hr; MOMO.OOO) and studied 48-72 hr 
after viral exposure. Phenotypic correction was assayed as the Cl" secretory 
response to forskolin measured in modified Ussing chambers. As can be seen in 
Figure 15, full correction of forskolin-stimulated Cl" currents is effected by the Ad5- 
CB-CFTR vector. 
Comparable studies have been performed on airway epithelial cells excised 
from CF subjects and grown on collagen matrices to form functioning monolayers. 
In a series of studies, CF monolayers have been exposed to Ad5-CB-CFTR 
(M01=10,000) for 24 hr and bioelectric characterization performed 3 days later. 
Summary data are shown in Figure 16 below: 
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