CF graft exposed to CFTR adenovirus demonstrated significant increases in PD in 
response to changes in luminal [C1‘] and forskolin (Figure 17, panel C). 
These xenografts have been explanted and evaluated for efficiency of 
genetically reconstituted cells. Previous experience indicates that CFTR expression 
should be detected in 5 - 20 % of the cells. Analyses of these grafts presented in 
Figure 17 are underway. These results indicate that single exposure of a CF 
epithelium to Ad5-CB-CFTR results in detectable differences in the bioelectric 
properties consistent with partial correction of CFTR function. 
IV.B. Safety 
IV.B.l. Studies of acute injuiy 
IV.B.l.a. Studies of potential inflammatory responses in mouse nasal 
and tracheal mucosa in response to topically applied Ad5-CMV- 
lacZ were performed. 
A series of studies designed to test whether Ad5-CMV-lacZ induced 
inflammatory responses in mouse airways have been initiated. A preliminary 
analysis of these studies is presented here. 
11 19 
Nasal cavities of mice were exposed to single (30 /zl, 10-10^ pfu/ml) 
superfusions of Ad5-CMV-lacZ. Nasal sections were resected three days later, 
stained for (3-gal, and evaluated for inflammatory cells (H&E). Representative 
samples of nasal mucosa are shown below (Figure 18). 
Figure 18. Nasal mucosa exposed to Ad5-CMV-lacZ and evaluated for lacZ 
expression and inflammatory cells. A: 100X. B: 400X. 
Recombinant DNA Research, Volume 17 
[473] 
