Sections from four animals exposed to Ad5-CMV-lacZ versus vehicle were 
read by a veterinary pathologist (D. Meuten - see Appendix G). No inflammatory 
infiltrate was noted in the nasal mucosa. 
Because of the more defined cylindrical surface area of the trachea, 
systematic studies were performed to evaluate the interaction between Ad5-CMV- 
lacZ superfusion and inflammatory cell infiltrates in the trachea. In an initial series 
of studies, the effects of a one-time exposure to Ad5-CMV-lacZ (30 /xl, 10^ 
pfu/ml) on the histologic features of mouse tracheas were studied (n=4). As in the 
nose, no inflammation was observed 3 days after Ad5-CMV-lacZ instillation. A 
similar series of studies was performed in which tracheas were infused three days 
consecutively with Ad5-CMV-lacZ and histologic evaluation performed two days 
after the final infusion (n = 2). Again, no inflammation was observed. Finally, a 
time course study (0, 1, 2, 3, 7, and 14 days) was performed to monitor for 
inflammation after a single exposure to Ad5-CMV-lacZ (30 /xl, 10^ pfu/ml);(n = 3 
mice/time point). Representative H&E sections are shown in Figure 19. No 
inflammation was observed in tracheas from animals sacrificed 1, 2, 3, and 5 days 
after instillation. Sparse focal distribution of mild inflammation was detected at day 
7, which appeared to diminish by day 14 (D. Meuten - see Appendix G). 
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Recombinant DNA Research, Volume 17 
