adenoviruses. Each animal was anesthetized, intubated, and ~ 3 ml of purified virus 
was administered into the airway through the endotracheal tube while the animal 
was lying in the left lateral decubitus position. Two animals (9037 and 9650) 
received Ad5-CMV-lacZ and one animal (8829) received Ad5-CB-CFTR. The 
animals were monitored clinically and serial blood hematology/chemistry were 
evaluated. Animals 9037 and 8829 were necropsied 3 days after administration of 
virus; animal 9650 will be necropsied 3 weeks post-infusion. A summary of the 
experiment is presented in the table below. 
Animal 
Virus 
Sex 
Wt (kz\ 
Type 
Total fpful 
9037 
Male 
3.6 
Ad5-CMV-lacZ 
3xlO U 
8829 
Male 
4.2 
Ad5-CB-CFTR 
3xlO U 
9650 
Male 
3.2 
Ad5-CMV-lacZ 
3xl0 n 
The animals tolerated the procedure well without obvious clinical sequelae. 
Necropsy evaluations of animals 9037 and 8829 three days after administration of 
virus failed to reveal pathology. Blood chemistry and hematology values remained 
within normal limits (see Tables in Appendix H). 
We have begun characterizing tissues from 9037 for evidence of adenovirus 
mediated gene transfer. Tissues from various organs were frozen, cryosectioned, 
mounted, and stained in X-gal. No staining was detected above background in 
brain, liver, spleen, kidney, heart, and testes. Significant staining was seen 
throughout the lung. Representative sections of lung are presented in Figure 20. 
There was patchy staining in proximal airway covering 1-2% of the surface and a 
majority of staining in the air space (alveolar cells and macrophages) with some 
reaction noted in distal airways. DNA/RNA analysis of all tissues is underway. 
[476] 
Recombinant DNA Research, Volume 17 
