X-gal histochemistry of terminal bronchiole (top) and alveolus (bottom) of 
monkey 9037. 
Figure 20. Expression of lacZ after Ad5-CMV-lacZ instillation into Rhesus 
monkey lung 
A similar experiment (B208) has been performed in a baboon (Papio anubis ). 
The specific aims were to: a) assess short term toxicity of virus administration, b) 
evaluate for the spread of virus beyond the lung, and c) determine the distribution 
and efficiency of transgene expression within the lung. In order to do this with any 
precision we decided to administer the virus and to necropsy the animal three days 
later. A lacZ virus was used to increase the sensitivity and maximize the precision 
of detecting transgene expression in organs. A 31 kg male baboon ( Papio anubis ) 
was anesthetized and 20 ml of 1 x 10^pfu/ml of Ad5-CMV-lacZ virus was instilled 
Recombinant DNA Research, Volume 17 
[477] 
