IV.B.5. Biology of recombinant adenovirus in human airway epithelia - 
including persistence, expression of adenoviral proteins, and recovery 
of adenovirus (see manuscript of Engelhardt et al., Appendix E) 
IV.B.5.a. Expression of late adenoviral proteins (hexon, fiber) within 
human bronchial epithelium 
Adenoviral protein expression from both wild type and recombinant 
adenovirus was analyzed by immunocytochemical techniques. Antibodies generated 
to purified adenovirus hexon protein (1056F) and purified adenovirus type 3 fiber 
protein (805F) have been shown to crossreact with all 43 human adenoviral 
serotypes. Both antibodies were directly labeled with FITC and used in double 
immunofluorescence studies with the polyclonal p-galactosidase antibody. Wild 
type adenoviral infection into xenografts produced immunoreactive cells with both 
adenoviral antibodies 805F (Figure 23D and E) and 1056F (data not shown) at 21 
hours postinfection. In contrast, retrovirally infected grafts showed no reactivity 
with these antibodies (Figure 23F and G). Xenografts infected with 10^ pfu/ml 
recombinant Ad5-RSV-lacZ adenovirus showed no evidence of adenoviral protein 
expression within 1,500 lacZ positive cells analyzed (Figure 23 A-C). 
»•* i 
,«*• I 
[484] 
Recombinant DNA Research, Volume 17 
