Immunocytochemical detection of adenoviral proteins. Frozen sections (6 jllm) of xenografts: 
panels A-C - infected with 10^ pfu/ml AdS-RSV-IacZ, harvested 3 days postinfection, and 
analyzed by double immunofluorescence (Nomarski, panel A) with antibodies to adenoviral 
proteins (805F, detected with FITC, panel B), and (3-galactosidase (detected with texas red, panel 
C; panels D and E-infected with wild type adenovirus, harvested 20 hours postinfection, and 
analyzed by double immunofluorescence (Nomarski, panel G) with antibodies to adenoviral 
proteins (805F, detected with FITC, panel F). Nuclear localized (3-galactosidase is marked by an 
arrow while an adenoviral protein positive cell is marked by a +. (bar = 30 /Lim). 
Figure 23. Immunocytochemical localization of adenoviral proteins in xenografts. 
IV.B.5.b. Expression of early and late adenoviral gene products in 
human bronchial epithelial xenografts 
We have developed techniques of triple immunofluorescence to colocalize 
the transgene product with a variety of adenoviral gene products including: a) the 
E2a gene expressed in the early and late phase of the adenoviral life cycle that 
encodes a 72 kd DNA binding protein; and b) the L3 and L5 transcripts, formed 
from the single late transcriptional unit, that encodes the structural proteins hexon 
and fiber, respectively. The program of adenoviral protein expression in cells of the 
human xenograft differed substantially between wild type Ad5 and the El deleted 
recombinants. Cells infected with wild type Ad5 expressed high levels of the 
Recombinant DNA Research, Volume 17 
[485] 
