Number of 
Ad5 viral molecules 
PCR assay for detection of wild type Ad5 virus particles. Total cellular DNA (125 ng) was 
supplemented with varying quantities of Ad5 DNA (0.1 to 100,000 Ad5 molecules/tube). These 
mixtures were subjected to 35 rounds of amplification with primers to Ela (5’-501 to 524, and 3’- 
1075 to 1099). Panel A shows agarose gel analysis of these reactions. An aliquot (2 fll) of the first 
round reactions were amplified another 35 cycles with nested primers to Ela (5’-527 to 548 bp and 
3’-1029 to 1053 bp). Agarose gel analysis of the second round PCR is shown in Panel B. 
Figure 25. PCR assay for wild-type adenovirus contamination. 
VII. OVERALL RISK/BENEFIT 
VILA. Risks to human subjects 
VII.A.1. Blood draws 
Localized hematoma at the puncture site- appropriate pressure will be 
applied to the puncture site to prevent this common complication. Vaso-vagal faint- 
patients will be asked to report any symptoms of lightheadedness or circumoral 
tingling which may precede a vaso-vagal episode, those individuals experiencing 
such symptoms will be kept in a recumbent position until this temporary reaction 
abates. If the above precautions are taken, the potential risk to blood donors is 
negligible. 
Recombinant DNA Research, Volume 17 
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