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Principal Invesligalor/Program Director (Last, first, middle): 
BIOGRAPHICAL SKETCH 
Give the following information for the key personnel and consultants and collaborators. Begin with the principal 
investigator/program director. Photocopy this page for each person. 
CVs 
NAME 
POSITION TITLE 
BARRANGER, JOHN A. 
Professor of Human Genetics 
EDUCATION (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) 
INSTITUTION AND LOCATION 
DEGREE 
YEAR 
CONFERRED 
FIELD OF STUDY 
Loyola College, Baltimore, MD 
B.S. 
1967 
Biology 
University of Southern California Graduate School 
University of Southern California 
Ph.D. 
1973 
Pharmacology 
School of Medicine 
M.D. 
1974 
Medicine 
RESEARCH AND PROFESSIONAL EXPERIENCE: Concluding with present position, list, in chronological order, previous employment. experience, and 
honors. Key personnel include the principal investigator and any other individuals who participate in the scientific development or exec-j'Jon of the project. 
Key personnel typically will include all individuals with doctoral or other professional degrees, but in some projects will include individual at the masters or 
baccalaureate level provided they contribute in a substantive way to the scientific development or execution of the project. Include present membership on 
any Federal Government public advisory committee. List, in chronological order, the titles, all authors, and complete references to all pub'cations during the 
past three years and to representative earlier publications pertinent to this application. DO NOT EXCEED TWO PAGES. 
1974-1976 
1976-1978 
1978-1986 
! 1983-1986 
1983-1986 
1986-1990 
1990-Present 
Intern and Resident, University of Minnesota, Department of Pediatrics 
Clinical Associate Development and Metabolic Neurology, RB, NINCDS, NIH 
Head, Clin. Investigations and Therapeutics Section, DMNB, NINCDS, NIH 
Acting Head, Molecular and Medical Genetics Section, DMNB, NINCDS, NIH 
Associate Chief, Development and Metabolic Neurology Branch, NINCDS, NIH 
Professor Pediatrics and Biochemistry, Univ. of Southern Cal., School of Medicine 
Professor Human Genetics; Molecular Genetics and Biochemistry; Pediatrics, University of Pittsburgh 
Honors : 1972 Phillip Aull Scholarship, 1973 Alpha Omega Alpha, 1973 March of Dimes Health Career Award, 1980 
USPHS Commendation Medal, 1984 Arthur S. Flemming Award; Society for Pediatric Research, 1987. 
Member : Biochemistry 2 Study Section 1986 - 1990; Chairman, Special Study Section: RFA on Gene Therapy, 1987; 1990, 
Co-Director, University of Pittsburgh, Gene Therapy Initiative; 1991, CHRC, Core Laboratory Director; 1992, 
Human Gene Therapy Applications Laboratory, Biotechnology Center, Director. 
Selected Bibliography 
Ginns, E.I., Choudary, P.V., Martin, B.M., Winfield, S., Stubblefield, B., Mayor, J., Markle-Lehman, D., Murray, G.J., 
Bowers, L.A. and Barranger, J.A. Isolation of cDNA clones for human glucocerebrosidase using the gt-11 expression 
system. Biochem. Biophys. Res. Commun. 123:574-580, 1984. 
Takasaki, S., Murray, G.J., Furbish, F.S., Brady, R.O., Barranger, J.A. and Kobata, A. Structure of the N-asparagine-linked 
oligosaccharide units of human placental glucocerebrosidase. J. Biol. Chem. 259: 10112-10117, 1984. 
Erickson, A.H., Ginns, E.E. and Barranger, J.A. Biosynthesis of the lysosomal enzyme, glucocerebrosidase. J. Biol. Chem. 
260:14319-14324, 1985. 
Ginns, E.I., Choudary, P.V., Tsuji, S., Martin, B., Stubblefield, B., Sawyer, J., Hozier, J. and Barranger, J.A. Gene mapping 
and leader polypeptide sequence of human gluocerebrosidase: Implications for Gaucher’s disease. Proc. Natl. Acad. Sci. 
USA 82:7101-7105, 1985. 
Tsuji, S., Choudary, P.V., Martin, B.M., Winfield, S., Barranger, J.A. and Ginns, E.I. Nucleotide sequence of cDNA containing 
the complete coding sequence for lysosomal glucocerebrosidase. J. Biol. Chem. 261:50-53,1986. 
Das, P.K., Murray, G.J. and Barranger, J.A. Studies on the turnover of glucocerebrosidase in cultured peritoneal macrophages and 
normal fibroblasts. Eur. J. Biochem. 154:445-450, 1986. 
Kaye, E.M., Ullman, M.D., Wilson, E.R. and Barranger, J.A. Type 2 and Type 3 Gaucher disease: A morphological and 
biochemical study. Ann. Neurol. 20:223-230, 1986. 
Choudary, P.V., Barranger, J.A., Tsuji, S., Mayor, J., LaMarca, M.E., Cepko, C.L., Mulligan, R.C. and Ginns, E.I. 
Retrovirus-mediated transfer of the human gluocerebrosidase gene to Gaucher fibroblasts. Mol. Biol. Med 3:293-299, 
1986. 
! Aerts, J.M.F.G., Donker-Koopman, W.E., Murray, G.J., Barranger, J.A., Tager, J.M. and Schram, A.W. A procedure for the 
rapid purificaiton in high yield of human glucocerebrosidase using immunoaffinity chromatography with monocolonal 
antibodies. Analyt. Biochem. 154:655-663, 1986. 
Willemsen, R., van Dongen, J.M., Sips, H.J., Reuser, A.J.J., Galjaard, H., Schram, A.W., Tager, J. M., Ginns, E.I. and 
Barranger, J.A. Ultrastructural localization of glucocerebrosidase in cultured Gaucher fibroblasts by immunocytochemistry. 
J. Neurol. 234:44-51, 1987. 
as 3a. 3b. 
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