FF Principal Investigator/Program Director (Last, first, middle): 
BIOGRAPHICAL SKETCH 
Give the following information for the key personnel and consultants and collaborators. Begin with the principal 
investigator/program director. Photocopy this page for each person. 
NAME POSITION TIT L^ ss j S (; an t Professor of 
Donald B. Kohn, M.D. Pediatrics and Microbiology 
EDUCATION (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) 
INSTITUTION AND LOCATION 
DEGREE 
YEAR 
CONFERRED 
FIELD OF STUDY 
University of Illinois, Urbana IL 
B.S. 
1976 
Biology 
University of Illinois, Urbana IL 
M.S. 
1978 
Microbiology 
University of Wisconsin, Madison, WI 
M.D. 
1982 
Medicine 
RESEARCH AND PROFESSIONAL EXPERIENCE: Concluding with present position, list, in chronological order, previous employment, experience, and 
honors. Key personnel include the principal investigator and any other individuals who participate in the scientific development or execution of the project 
Key personnel typically will include all individuals with doctoral or other professional degrees, but in some projects will include individuals at the masters or 
baccalaureate level provided they contribute in a substantive way to the scientific development or execution of the project. Include present membership on 
any Federal Government public advisory committee. List, in chronological order, the titles, all authors, and complete references to all publications during the 
past three years and to representative earlier publications pertinent to this application. DO NOT EXCEED TWO PAGES. 
PROFESSIONAL EXPERIENCE 
Pediatric Internship and Residency, University of Wisconsin Hospital, Madison, Wisconsin, 1982-85. 
Medical Staff Fellow, Metabolism Branch, National Cancer Institute, National Institutes of Health (NIH), 
Bethesda, Maryland, 1985-87. 
ACADEMIC APPOINTMENTS 
Attending Physician, Bone Marrow Transplantation, Childrens Hospital of Los Angeles. 
Assistant Professor, Departments of Pediatrics and Microbiology, University of Southern California School of Medicine, 1988- 
Present. 
HONORS 
Selected by the Awards Committee of the Western Society for Pediatric Research as the 1993 recipient of the Ross Young 
Investigator Award. 
PUBLICATIONS 
Peer Review Articles 
1. Kohn DB, Weber MJ, Carl PL, Katzenellenbogen JA, Chakravarty PK. Peptidyl derivatives of (3H)-aniline as sensitive stable 
protease substrates. Anal Biochem 97:269-76, 1979. 
2. Kantoff PW., Kohn DB, Mitsuya H, Armentano D, Sieberg M, Zwiebel JA, Eglitis MA, McLachlin JR, Wiginton DA, Hutton JJ, 
Horowitz SD, Gilboa E, Blaese RM, Anderson WF. Correction of adenosine deaminase deficiency in human T and B cells 
using retroviral gene transfer. Proc Nat Acad Sci USA 83:6563-67, 1987. 
3. Kohn DB, Trigg MD, Borcherding W, Hong R. Immunologic studies of lymph node lymphocytes in the generalized 
lymphadenopathy syndrome. Am J Ped Hem One 9:1-7, 1987. 
4. Kantoff PW, Gillio A, McLachlin JR, Bordignon C, Eglitis MA, Kernan NA, Moen RC, Kohn DB, Yu S, Karson E, Karlsson S, 
Zweibel JA, Gilboa E, Blaese RM, Nienhuis A, O’Reilly RJ, Anderson WF. Expression of human adenosine deaminase in non- 
human primates after retroviral mediated gene transfer. J Exp Med 166:210-34, 1987. 
5. Kohn DB, Kantoff PW, Eglitis M, McLachlin JR, Moen RC, Karson E, Zwiebel JA, Nienhaus A, Karlsson S, O’Reilly R, Gillio 
A, Bordignon C, Gilboa E, Zanjani ID, Blaese RM, Anderson WF. Retrovirus-mediated gene transfer into mammalian cells. 
Blood Cells 13:285-96, 1987. 
6. Eglitis MA, Kohn DB, Moen RC, Blaese R, Anderson WF. Infection of human hematopoietic progenitor cells using a retroviral 
vector with a xenotropic pseudotype. Biochem Biophys Res Comm 151:201-06, 1988. 
7. Fearon ER, Kohn DB, Winkelstein JA, Vogelstein B, Blaese RM. Carrier detection in the Wiskott-Aldrich syndrome. Blood 
72:1735-39, 1988. 
8. Kohn DB, Mitsuya H, Ballow M, Selegue JE, Barankiewicz J, Cohen A, Gelfand E, Anderson WF, Blaese RM. Establishment 
and characterization of adenosine deaminase (ADA)-deficient human T cell lines. J Immunol 142:3971-77, 1989. 
9. Nolta JA, Sender LS, Barranger JA, Kohn DB. Expression of human glucocerebrosidase in murine long-term bone marrow 
cultures after retroviral vector-mediated transfer. Blood 75:787-97, 1990. 
lO.Ozkaynak MF, Lenarsky C, Kohn D, Weinberg K, Parkman R. Mycobacterium avium -intracellular infections after allogeneic 
bone marrow transplantation in children. Am J Ped Hematol-Oncol 12:220-24, 1990. 
ll. Nolta JA, Kohn DB. Comparison of the effects of growth factors on retroviral vector-med iated gene transfe r and the 
PHS 398 (Rev. 9/91) (Form Page 6) Page 
Number p^g^psecutively at the bottom throughout the application. Do nofuse suffixes DNA Research, Volume 17 
