Galpin/DA/N2 IIIBenv 
TABLE OF CONTENTS 
SCIENTIFIC ABSTRACT OF THE STUDY 2 
NON-TECHNICAL ABSTRACT OF THE STUDY 4 
1 .0 OBJECTIVES AND RATIONALE OF THE RESEARCH 5 
2.0 VECTOR CONSTRUCT, PACKAGING AND PRODUCER CELL LINES 8 
2. 1 VECTOR CONSTRUCT 8 
2.1.1 Sequence Analysis of the Vector 9 
2.2 PACKAGING CELL LINE 9 
2.2. 1 Parent Cell Line 9 
2.2.2 Construction of Packaging Cell Line 10 
2.3 GENERATION OF THE PRODUCER CELL LINE (DA/N2 IIIBenv) 
USED TO GENERATE HIV-IT (V) 10 
2.3. 1 Characterization of DA/N2 IIIBenv. 2C3 Producer Cell Line 
(PCL) 12 
2.3.2 Growth Conditions of the Producer Cell Line and Purification of 
HIV-IT (V) 13 
2.3.3 Safety Testing of Producer Cell Line 13 
2.3.4 Safety Testing of the Vector, HIV-IT (V) 13 
2.3.5 Safety Features of the Producer Cell Line 14 
3.0 PRECLINICAL STUDIES 17 
3.1 MOUSE MODEL 18 
3.1.1 CTL Induction By HIV-IT (V) 18 
3. 1 . 1 .2 Dose Response 1 9 
3. 1.1. 3 HIV-IT (V) Encoded Proteins Induce the CTL Response 19 
3. 1 . 1 .4 Phenotype of Cytotoxic Effector Cells 20 
3. 1 . 1 .5 HIV- 1 env/rev Specificity of CTL 20 
3. 1 . 1 .6 Ability of Induced CTL to Lyse Cells Infected with 
Divergent HIV-1 Strains 22 
3.1.2 Antibody Induction 24 
3.1.3 Conclusion 25 
Recombinant DNA Research, Volume 17 
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