Galpin/DA/N2 IIIBenv 
analysis of potential clinical cross-coverage of cells infected with different HIV- 1 
strains. 
In previous studies, the HIV-IT (V) transduced syngeneic fibroblast cell 
line (BCenv) has been used to induce HIV- 1IIIB env-specific CTL in B ALB/c 
mice. CTL crossreactivity was then evaluated on Hu/D d target cells infected with 
different HIV-1 isolates. BCenv-induced CTL were analyzed for their ability to 
lyse target cells infected with different HIV-1 prototypic strains as well as HIV-1 
clinical isolates obtained from HIV- 1 -infected patients. These CTL were able to 
lyse Hu/D d target cells infected with the HIV-1 IIIB, HIV-1 MN, HIV-1 SF2, 
HIV-1 WMJII, and HIV-1 CC prototypic viral isolates, but not uninfected Hu/D d 
control targets (Figure 13) 62 . Interestingly, the Hu/D d cells infected with HIV-2 
were not lysed suggesting that HIV-2 infected cells do not express CTL epitopes 
shared with HIV-1 isolates. Furthermore, BCenv-induced CTL were capable of 
lysing Hu/D d target cells infected with several clinical isolates of HIV-1 obtained 
from HIV-infected patients (Figure 14A), as well as sequential HIV-1 clinical 
isolates that have become resistant to AZT (Figure 14B). 
The results show that CTL induced with HIV-IT (V)-transduced cells have 
a broad reactivity against a number of HIV-1 prototypic strains and clinical isolates. 
These results also suggest that the range of CTL reactivity is less restricted than that 
seen for type-specific antibody responses perhaps due to recognition of conserved 
epitopes by CTL effectors. Therefore, the Hu/D d target cell system has 
demonstrated that CTL induced by cells transduced with a retroviral vector 
encoding HIV-1 IIIBenv, are capable of recognizing diverse strains of HIV-1. 
3.1.2 Antibody Induction 
The antibody response, specific for the HIV-1 env protein (i.e., gp 160/1 20) has 
been examined in mice. Following administration of HIV-IT (V). Shown in Figure 15 
^ 2 Chada, et al., J. Virol., (1993) In Press. 
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