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APPENDIX D TO APPENDIX C 
committees which we expect will have to be Involved, we must 
take measures to minimize potential risks. NIH is at present 
preparing regulations concerning procedures for review of new 
projects, but we also must assure ourselves about work being 
done or to be done under grants about to be av;arded or under 
existing grants. Accordingly, v;e require from you a statement 
assuring the National Cancer Institute that techniques considered 
appropriate for the estimated risks are being or will be applied. 
The information required is detailed in the following sample 
statement : 
This proposed research project involved the synthesis 
of recombinant DNA molecules betv;een: (specify details). 
The carrier agent to be employed is: (specify details), 
and the principal investigator evaluates its risk level 
as: * (minimal, low, moderate, or high with basis of 
assessment specified). 
The laboratory where the research will be performed will 
practice rigorously, medical bacteriological procedures 
including sterilization of all biological materials before 
disposal. All of the personnel associated with this 
research are aware of the potential risks involved and 
have been properly trained and instructed on the importance 
to themselves and others of practicing proper laboratory 
procedures . 
In addition, the principal investigator is aware of the 
principles and guidelines for containment proposed at 
the Asilomar Conference dealing with cell-free generated 
recombinant DNA molecules, the standards of biological 
safety and environmental control published by the National 
Cancer Institute, and the lab safety guide of the Center 
for Disease Control. The proposed research will comply 
with these principles and standards by using the following 
safety procedures: (specify details regarding pertinent 
lab practices and the use of appropriate physical and 
biological safeguards) . 
The research participants will use safer vectors and host 
cells as soon as they are developed but understand that 
no change of biological material or procedures that may 
affect the biohazard aspects of the proposed research will 
be made without prior NIH approval. It is further 
understood that preliminary or pilot experiments using 
Appendix C — 17 
