APPENDIX D TO APPENDIX C 
to utilize clones constructed under Asilomar guidelines in systems 
v^ich provide the level of biological containment specified in the 
in the NIH guidelines. Investigators should contact the NIH Office 
of Recombinant ENA Activities for further information. 
Review and Certification of Ongoing Projects 
At the present time the institution must submit a Memorandum of 
Understanding and Agreement (MUA) for each ongoing project involving 
recombinant ENA technology. The MUA must include: 
1. An assurance that the principal investigator has become 
familiar with the guidelines issued by the NIH on 
June 23 and published in the Federal Register on 
July 1 , and agrees to abide by their provisions, 
2. A description of the experiments being conducted which 
involve recombinant ENA molecules, 
3. An assessment of the levels of physical and biological 
containment required by the current NIH guidelines for 
these experiments, 
4. A statement of the facilities and specific procedures 
which will be used to provide the required levels of 
containment , 
5. Certification by an institutional official that these 
facilities and procedures have been reviewed by the 
institutional biohazards committee, and judged to be 
both adequate and consistent with the requirements of 
the NIH guidelines, 
6. Agreement that the institutional biohazards committee 
will monitor the facilities and procedures throughout 
the duration of the project, and 
7. A statement that the recombinant DNA molecules being 
used will not be transferred to other investigators 
or institutions unless their facilities and techniques 
have been assured to be adequate, and a new MUA is 
executed by them and submitted to the NIH prior to 
the initiation of experiments. 
Appendix C — 22 
