APPENDIX E 
Table 3. Guide! ines for experiments with col i 
host-vector systems in which the foreign DNA 
is from an extrachromosoma I element 
Conta i nment 
"Fore i gn" 
DNA source 
Phys i ca 1 
B 
iol og ical 
Animal viruses 
P4 
or 
EK2 
P3 
EK3 
1 f c 1 oned , free of 
harmful genes 
P3 
EK2 
P 1 ant V i ruses 
P3 
or 
EK1 
P2 
EK2 
a/ 
Eukaryote organelles 
Pr i mates 
P3 
or 
EK1 
P2 
EK2 
Other 
P2 
EK1 
Prokaryote (plasmids and phage) 
"Exchangers" 
Non-pathogens 
P1 
EK1 
Pathogen 
As for 
shotgun 
"Non-exchangers" 
As for 
shotgun 
The organelle DNA must be purified from isolated organelles. Otherwise 
conditions indicated for shotgun experiments apply. 
Appendix E — 29 
