APPENDIX G 
ONGOING NIH-SUPPORTED PROJECTS INVOLVING RECOMBINANT DNA, 
WITH MEMORANDA OF UNDERSTANDING AND AGREEMENT (MUA) , 
ON FILE WITH THE NIH OFFICE OF RECOMBINANT DNA ACTIVITIES 
A. Intramural Projects (as of July 25, 1977 ) 
Physical 
Containment 
Institute Description Level 
NIAID IMUA-10 - Cloning of DNA from E. coli K-12 in mutants PI 
and derivatives of E. coli K-12 using either an EK2 
lambda phage or nonconjugative plasmid vector. 
NIAMDD IMUA-4 - Examination of the genetic composition of Pi 
Col El - E. coli DNA hybrids in various colonies by 
standard mating techniques with F~E . coli . 
IMUA-6 - Study of a DNA fragment from a restriction Pi 
endonuclease digest of total E. coli DNA inserted 
into the nontransmissable plasmid Col El. 
IMUA-7 - Study of DNA sequences which engage in site- PI 
specific recombination events. Analysis of recombinants 
of bacteriophage (|> 80d3 ilv + su-?'*’ DNA fragments and Eco 
Rl-treated DNA of the plasmid vector pSClOl for the 
presence of the sequence in chimeric plasmids. 
IWUA-15 - Study of integrative recombination of Pi 
bacteriophage lambda. 
IM UA-2 0 - Study of the yS sequence of the F plasmid of Pi 
E. coil K-12, in particular the genetic (functional and 
structural) requirements of _ specific reccrabination. 
IMUA-21 - Study of the mechanism of initiation of the PI 
E. coli genome by construction of a hybrid plasmid 
containing Col El and a piece of the E. coli gencxne 
which contains the E. coH origin of replication. 
IMUA-25 - Cloning and purification of DNA Ligase in E. coli PI 
NCI IMUA-9 - Cloning of a number of E . coli and bacteriophage Pi 
IWA se^ents in plasmid DNA vectors using E. coli K-12 
as a host. 
IMUA-13 - Initiation and control of transcription in SV40. P3 
IMUA-16 - Effect of steroid hormones on the transcription P3 
of the vitellogenin and albumin genes in chickens. 
IMUA-17 - Study of the organization and expression of P2 
yeast genes. 
Appendix G--1 
