Protocol Flow Sheet 
HIV-negative Donor with HIV-Positive Identical Twin 
I 
T 
Apheresis of Donor 
I 
T 
CD8-f and CD4" T Cell selection 
I 
T 
Genetic modification (retroviral transduction) inserting CD4-zeta 
(cohorts 1, 2, 3 only) 
I 
▼ 
Select genetically modified T cells by CD4-I- selection 
I 
T 
Primary ex vivo T cell expansion 
I 
T 
Cryopreserve cell stock 
I 
T 
Secondary ex vivo cell expansion 
I 
T 
Cell harvest for infusate preparation 
I 
T 
Infuse into HIV-Positive Twin 
I 
T 
Monitor safety and immunological parameters 
Recombinant DNA Research, Volume 19 
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