Protocol 
RAC Application 
Kenneth L. Brigham, M,D. 
References from our laboratories 
PI. Schreier, H., Gonzalez-Rothi, R. Stecenko, A. Pulmonary delivery of liposomes. J 
Control Release 24:209-223. 1993. 
P2. Schreier, H., McNicol, K., Ausborn, M., Soucy, D., Derendorf, H., Stecenko, A., Gonzalez- 
Rothi, J. Pulmonary delivery of amikacin liposomes and acute liposome toxicity in the 
sheep. Int J Pharmaceut 87:183-193. 1992. 
P3. Myers, M.A., Thomas, D.A., Straub, L., Soucy, D.W., Niven, R.W., Kaltenbach, M., Hood, 
C.I., Schreier, H., and Gonzalez-Rothi, R.J. Pulmonary Effects of Chronic Exposure to 
Liposome Aerosols in Mice. Experimental Lung Research 19:1-19, 1993. 
P4. Brigham, K., Meyrick, B., Christman, B., Magnuson, M., King, G., Berry, L. Iii vivo 
transfection of murine lungs with a functioning prokaryotic gene using a liposome 
vehicle. Am J Med Sci 296:278-281. 1989. 
P5. Brigham, K., Canonico, A., Conary, J. Mediator gene expression in sepsis: Implications 
for therapy. In: Update in Intensive Care and Emergency Medicine, Volume 16: 
Mediators of Sepsis Sprineer-Verlae. 1992, pp. 393-405. 
P6. Brigham, K., Canonico, A., Mcyrick, B., Conary, J., Schreier, H. Prospects for gene 
therapy for endotoxin induced lung injury. In: Endotoxin and the Lungs K. Brigham, 
Ed., Marcel Dekker, Inc. Accepted for publication, 1993. 
P7. Brigham, K., Canonico, A., Meyrick, B., Schreier, H., Stecenko, A., Conary, J. Gene 
therapy for inflammatory diseases. In Bacterial Endotoxins: Basic Science to Anti- 
Sepsis Strategies : Proceedings of the International Conference on Endotoxins IV, 
Amsterdam, 1993. J. Levin, A. Sturk, T. van dcr Poll, S.J.H. vanDcvcnter, eds., John 
Wiley and Sons, New York. Accepted for publication, 1993. 
P8. Brigham, K., Mcyrick, B., Christman, B., Conary, J., King, G., Berry, L., Magnuson, M. 
Expression of human growth hormone fusion genes in cultured lung endothelial cells 
and in the lungs of mice. Am J Resp Cell Mol Biol 8:209-213, 1993. 
P9. Canonico, A., Conary, J., Mcyrick, B., Brigham, K. Cationic liposome mediated transfer 
of human alpha-1 antitrypsin gene to lungs of rabbits. Am J Resp Cell Mol Biol In 
press, 1993. 
PIO. Canonico, A., Conary, J., Christman, B., Mcyrick, B., Brigham, K. Expression of a CMV 
promoter driven human a-1 antitrypsin gene in cultured lung endothelial cells and in 
the lungs of rabbits. Clin Res 39:2 19 A, 1991. 
PI I. Canonico, A., Plitman, J., Conary, J., Meyrick, B., Brigham, K. Repeated intravenous or 
aerosol administration of plasmid-liposome complexes causes prolonged expression of 
human alpha-1 antitrypsin in rabbit lung without toxicity. J Appl Physiol Submitted, 
1993. 
P12. Plitman, J., Canonico, A., Conary, J., Mcyrick, B., Brigham, K. The effects of inhaled 
and intravenous DNA/liposomes on lung function and histology in the rabbit. Am Rev 
Resp Pis 145:A371, 1992. 
P13. Conary, J., Parker, R., Christman, B., Faulks, R., King, G., Brigham, K. Protection of 
rabbit lungs from endotoxin injury by in. vivo hyperexpression of the prostaglandin 
G/H synthase gene. J Clin Invest Under revision, 1993. 
PI4. Conary, J., Parker, R., Christman, B., Faulks, R., King, G., Brigham, K. Alteration of 
the endotoxin response by expression of a CMV promoter driven ovine prostaglandin 
G/H synthase gene in the lungs of rabbits. Am Rev Resp Pis 147:A546, 1993. 
P15. Brigham, K., Canonico, A., Conary, J., Mcyrick, B., Schreier, H. Potential for gene 
therapy in the treatment of sepsis. Chapter in the Scries: "Update in Intensive Care and 
Emergency Medicine". J Vincent, ed., Springer-VcrIag. Accepted for publication, 1993. 
P16. Brigham, K., Woolvcrton. W., Blake, L., Staub, N. Increased sheep lung vascular 
permeability caused by Pseudomonas bacteremia. J Clin Invest 54:792-804, 1974. 
P17. Brigham, K., Schreier, H. Cationic liposomes and DNA delivery. J Liposome Res 3:31- 
49, 1993. 
Recombinant DNA Research, Volume 19 
[183] 
