Protocol 
RAC Application 
Kenneth L. Brigham, M.D. 
30. Chomczynski, P., Sacchi, N. Single-step method of RNA isolation by acid guanidinium 
thiocyanatc-phcnol-chloroform extraction. Anal Biochem 162:156-159. 1987. 
31. Read, S., Northcote, D. Minimization of variation in response to different proteins of 
the coomasic blue G dye-binding assay for protein. Analvt Biochem 1 16:53-64, 1981. 
32. Gallagher, S., Smith, J. Electrophoretic separation of proteins. In: Current Protocols in 
Molecular Biology . Ausubcl, F., Brent, R., Kingston, R., Moore, D., Seidman, J., Smith, 
J., Struhl, K., cds. Greene Publishing and Wilcy-Intcrscicnce, New York, 10.2.1-10.2.21. 
33. Gallagher, S., Winston, S., Fuller, S., Hurrcll, J. Immunoblotting and immunodetection. 
In: Current Protocols in Molecular Biology . Ausubcl, F., Brent, R., Kingston, R., Moore, 
D., Seidman, J., Smith, J., Struhl, K., cds., Greene Publishing and Wiley-Interscience, 
New York. 10.8.1-10.8.16. 
Recombinant DNA Research, Volume 19 
[191] 
